Opportunity Information: Apply for P19AC00092
The grant opportunity titled "Monitoring Stream Benthic Macroinvertebrates for NPS-NCR" is a National Park Service (NPS) cooperative agreement focused on springtime biological monitoring of streams in the National Capital Region. The central purpose is to implement an established stream monitoring protocol (Norris and Sanders, 2009) and generate standardized data that can be used to evaluate and track stream health over time. Work is conducted across a relatively large footprint for a seasonal monitoring effort: thirty-seven stream sampling locations distributed among ten NPS parks in the National Capital Region.
The cooperator is responsible for carrying out field sampling and observations at each site, covering multiple lines of evidence that together describe stream condition. This includes faunal observations beyond insects, specifically noting herpetofauna (amphibians and reptiles), mussels, and crayfish when encountered. The core biological dataset comes from benthic macroinvertebrate sampling, which involves collecting organisms that live on or within the stream bottom sediments and are widely used as indicators of water quality and habitat condition. In addition to the biological collections, the work also requires completing a physical habitat assessment at each site and collecting water chemistry data, creating a combined dataset that links biological response to environmental conditions.
A notable component of this opportunity is the collection of additional samples intended for later DNA analysis. The agreement covers the collection and initial processing of the water samples for that purpose, but it explicitly does not cover DNA sequencing itself. Sequencing costs and laboratory processing for the genetic work are described as outside the scope of this award and would be funded through a separate agreement or a later modification. In practice, that means the cooperator must follow proper collection, handling, and initial processing steps so the samples remain usable for subsequent genetic analysis even though the sequencing will be handled under a different funding action.
For the traditional macroinvertebrate work, the cooperator must collect and preserve benthic samples for laboratory identification. The identification requirement is to classify specimens to the finest practical taxonomic resolution, with a target of genus-level identification. Using those identifications, the cooperator will calculate the Benthic Index of Biotic Integrity (BIBI) for each sampling location, relying on the most current methods used by the Maryland Biological Stream Survey Program. The BIBI is a commonly used bioassessment tool that converts macroinvertebrate community information (such as composition, diversity, and pollution tolerance) into a standardized index that supports comparisons among sites and across years.
Data handling and quality control are emphasized as major deliverables. All collected information, including photographs, must be entered into an MS Access database supplied by the NPS. Field crew members are expected to thoroughly check data during entry, and then the entire dataset must go through a 100 percent verification step by someone other than the original data enterer, ensuring a full independent review rather than a partial audit. At the end of the field season, the complete package of materials, including the database, photographs, and original data sheets, must be transferred to the National Capital Region Network (NCRN) Data Manager for long-term stewardship and use in the monitoring program.
After fieldwork concludes, the cooperator must produce a summary of monitoring activities that documents what was completed and highlights any interesting, unusual, or novel observations made during the season. The cooperator also participates in an Operational Review meeting, typically lasting one to two hours, which functions as a debrief covering safety performance, field procedures, and data management. The opportunity description stresses that cooperator feedback is an important part of maintaining a safe, consistent, and successful monitoring program over time.
Administratively, the opportunity is a discretionary cooperative agreement issued by the Department of the Interior, National Park Service, under CFDA 15.945, categorized under Science and Technology and other Research and Development. Eligible applicants are limited to public and state-controlled institutions of higher education, and the announcement anticipated a single award with an award ceiling of $44,367. The posting dates indicate it was created on April 5, 2019, with an original closing date of April 15, 2019, reflecting a short application window consistent with seasonal field monitoring timelines.Apply for P19AC00092
- The Department of the Interior, National Park Service in the science and technology and other research and development sector is offering a public funding opportunity titled "Monitoring Stream Benthic Macroinvertebrates for NPS-NCR" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.945.
- This funding opportunity was created on Apr 05, 2019.
- Applicants must submit their applications by Apr 15, 2019. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- Each selected applicant is eligible to receive up to $44,367.00 in funding.
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Public and State controlled institutions of higher education.
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Frequently Asked Questions (FAQs)
What is the title of this grant opportunity?
The opportunity is titled "Monitoring Stream Benthic Macroinvertebrates for NPS-NCR."
What type of funding opportunity is this?
This is a discretionary cooperative agreement issued by the Department of the Interior, National Park Service (NPS).
What is the main purpose of this cooperative agreement?
The central purpose is to conduct springtime biological monitoring of streams in the National Capital Region using an established protocol (Norris and Sanders, 2009) and generate standardized data that can be used to evaluate and track stream health over time.
Where will the work take place?
Work is conducted in the National Capital Region across thirty-seven stream sampling locations distributed among ten NPS parks.
When is the monitoring performed?
The monitoring is described as springtime biological monitoring, and it is framed as a seasonal field monitoring effort.
How many stream sites are included in this effort?
There are thirty-seven stream sampling locations included.
How many parks are involved?
The sampling locations are distributed among ten National Park Service parks in the National Capital Region.
What monitoring protocol is used?
The cooperator is expected to implement an established stream monitoring protocol referenced as Norris and Sanders (2009).
What is the cooperator responsible for doing in the field?
The cooperator is responsible for carrying out field sampling and observations at each site and collecting multiple lines of evidence describing stream condition, including biological sampling, physical habitat assessment, and water chemistry data collection.
What is meant by "multiple lines of evidence" for stream condition?
Based on the opportunity description, multiple lines of evidence include benthic macroinvertebrate sampling, physical habitat assessment, water chemistry data, and field observations of other animals encountered (such as amphibians, reptiles, mussels, and crayfish).
What biological data are collected as the core dataset?
The core biological dataset comes from benthic macroinvertebrate sampling, which collects organisms living on or within stream-bottom sediments and uses them as indicators of water quality and habitat condition.
Are observations required for organisms other than insects?
Yes. Field crews are expected to note faunal observations beyond insects, specifically including herpetofauna (amphibians and reptiles), mussels, and crayfish when encountered.
What additional non-biological data are collected at each site?
In addition to biological collections, the cooperator must complete a physical habitat assessment and collect water chemistry data at each site.
How is the biological information used to evaluate stream health?
Identified macroinvertebrate data are used to calculate the Benthic Index of Biotic Integrity (BIBI) for each sampling location, supporting standardized comparisons among sites and across years.
What is the Benthic Index of Biotic Integrity (BIBI) in this project?
The BIBI is described as a bioassessment tool that converts macroinvertebrate community information (including composition, diversity, and pollution tolerance) into a standardized index used to compare stream condition among locations and over time.
Which methods are used for calculating the BIBI?
The cooperator will calculate BIBI values using the most current methods used by the Maryland Biological Stream Survey Program.
What taxonomic identification level is required for macroinvertebrates?
Benthic samples must be preserved for laboratory identification, and specimens should be classified to the finest practical taxonomic resolution, targeting genus-level identification.
Does the award include DNA sequencing for genetic analysis?
No. The award includes collection and initial processing of water samples intended for later DNA analysis, but DNA sequencing and laboratory processing for the genetic work are explicitly outside the scope of this award.
What DNA-related work is included in the scope of this award?
The cooperator is responsible for collecting and initially processing the water samples so they remain usable for later DNA analysis. Sequencing would be funded separately through another agreement or a later modification.
What are the data management requirements for this project?
All collected information, including photographs, must be entered into an MS Access database supplied by NPS.
What quality control steps are required for data entry?
Field crew members are expected to thoroughly check data during entry. After entry, the entire dataset must undergo 100 percent verification by someone other than the original data enterer, providing a full independent review.
What materials must be delivered at the end of the field season?
At the end of the field season, the complete package must be transferred to the National Capital Region Network (NCRN) Data Manager, including the MS Access database, photographs, and original data sheets.
Who receives the final dataset and project materials?
The National Capital Region Network (NCRN) Data Manager receives the final database and supporting materials for long-term stewardship and use in the monitoring program.
Is a written report required after fieldwork concludes?
Yes. After fieldwork, the cooperator must produce a summary of monitoring activities documenting what was completed and highlighting any interesting, unusual, or novel observations made during the season.
Is there a meeting requirement as part of the project?
Yes. The cooperator participates in an Operational Review meeting, typically lasting one to two hours, to debrief safety performance, field procedures, and data management.
What topics are covered in the Operational Review meeting?
The Operational Review functions as a debrief and covers safety performance, field procedures, and data management. The opportunity also notes that cooperator feedback helps maintain a safe, consistent, and successful monitoring program.
Who is eligible to apply?
Eligible applicants are limited to public and state-controlled institutions of higher education.
What is the CFDA number associated with this opportunity?
The CFDA number listed is 15.945.
How is this opportunity categorized?
It is categorized under Science and Technology and other Research and Development.
How many awards were anticipated?
The announcement anticipated a single award.
What is the award ceiling for this cooperative agreement?
The award ceiling is $44,367.
When was the opportunity posted and when did it close?
The posting indicates it was created on April 5, 2019, and the original closing date was April 15, 2019.
What does the short application window suggest about the timeline?
The description notes that the short window is consistent with seasonal field monitoring timelines, implying the project is aligned to spring fieldwork scheduling.
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